Figure 2.
Generation of iRBCs. (A) Schematic of 12-day erythroid liquid culture of iPSC-derived HPCs into mature iRBCs. (B) Representative flow cytometric analysis of common cell surface erythroid maturation markers on day 12 of ce (rr) iRBC culture compared with control dRBCs. (C) Morphology of control dRBCs and day 12 ce iRBCs (May-Grunwald Giemsa stain). (D) Size distribution of day 12 ce iRBCs compared with control dRBCs measured by Countess Automated Cell Counter (n = 205 and 1435, respectively, Welch’s t test). (E) Fold expansion of iRBC per fresh HPC in erythroid-specific culture on days 3, 6, 9, and 12 (n = 22 independent assays). Ery, erythroid; IMDM, Iscove’s modified Dulbecco medium; pen strep, penicillin-streptomycin; Epo, erythropoietin, SCF, stem cell factor.

Generation of iRBCs. (A) Schematic of 12-day erythroid liquid culture of iPSC-derived HPCs into mature iRBCs. (B) Representative flow cytometric analysis of common cell surface erythroid maturation markers on day 12 of ce (rr) iRBC culture compared with control dRBCs. (C) Morphology of control dRBCs and day 12 ce iRBCs (May-Grunwald Giemsa stain). (D) Size distribution of day 12 ce iRBCs compared with control dRBCs measured by Countess Automated Cell Counter (n = 205 and 1435, respectively, Welch’s t test). (E) Fold expansion of iRBC per fresh HPC in erythroid-specific culture on days 3, 6, 9, and 12 (n = 22 independent assays). Ery, erythroid; IMDM, Iscove’s modified Dulbecco medium; pen strep, penicillin-streptomycin; Epo, erythropoietin, SCF, stem cell factor.

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