Long-term exposure to TCRvβ-CARTs does not result in bidirectional killing or proliferation of patient cancer cells. (A) Schematic of the experimental setup. CD8⁺ effector cells were produced to be distinguished from the CD4⁺ target patient cells, and both populations were analyzed for cell count and Ki67 expression at the end of a 5-day coculture. (B) Dot plots of the target patient (orange) and effector CART (gray) cell populations after 5 days of coculture. (C) Quantified cell counts and (D) Ki67 staining of identified cells at the end of the coculture. Data are representative of experiments repeated 3 times with CARTs derived from different healthy donors (∗P < .0332, ∗∗P < .0021, ∗∗∗P < .0002, ∗∗∗∗P < .0001 by one-way ANOVA with Dunnett multiple comparison test). Data for all figures are representative of experiments repeated 3 times with CARTs derived from different healthy donors.