Patient population cumulative coverage by shared GVL mHAs. (A) Coverage of DISCOVeRY-BMT patients with HLA-A∗02:01 allele with predicted GVL mHAs. Noncumulative independent population frequencies of each of the top 15 peptides within the HLA-A∗02:01 population range from 19.4% to 28.3%, shown as bar heights. The colors of the bars show z-scores of expression for the genes that contain each peptide from The Cancer Genome Atlas AML sample expression data (TCGA_AML). Cumulative population coverage by the 15 predicted GVL mHAs needed to reach 100% corresponding coverage is shown as an overlaid line graph. Dotted lines indicate 7 peptides needed to reach 90% population coverage. (B) Shows coverage of DISCOVeRY-BMT patients with HLA-B∗35:01 allele with predicted GVL mHAs. Eleven predicted GVL mHAs correspond to 100% cumulative population coverage and 6 correspond to 90% coverage for this HLA allele. Noncumulative coverage by the top 11 peptides for this HLA allele range from 20.9% to 29.3%. (C) Shows coverage of DISCOVeRY-BMT patients with HLA-C∗07:02. Fourteen predicted GVL mHAs correspond to 100% cumulative population coverage and 7 correspond to 90% for this allele. Noncumulative coverage for these mHAs ranges from 19.3% to 31.1%.