Iron-depleted MPN mice show accumulation of megakaryocyte-committed progenitors in the spleen. (A) Analysis of hematopoietic progenitor frequencies in the BM and spleen at terminal workup. Groups of 4 to 8 mice per genotype and treatment were analyzed. (B) Analysis of erythroid maturation. Frequencies of the erythroid maturation stages in the BM and spleen are shown for individual genotypes (n = 4-8 mice per genotype and treatment). All data are presented as mean ± standard error of the mean. Two-way ANOVAs with subsequent Dunnett posttest were used for multiple-group comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. CFU-E, colony-forming unit erythroid.