Status of iron parameters at the time of diagnosis in the serum of patients with MPN having mutations in JAK2 gene. (A) Iron parameters in the serum of patients with MPN at the time of diagnosis. (B) Levels of regulatory cytokines involved in iron metabolism measured in the serum of patients with MPN at the time of diagnosis. All data are presented as mean ± standard error of the mean. Two-way analyses of variance (ANOVAs) with subsequent Tukey test were used for multiple-group comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. NC, normal control; sTfR, soluble transferrin receptor, VAF, variant allele frequency.