Figure 7.
Pluripotin induces apoptosis in leukemic cells while enriching the normal HSC progenitors. (A) Scatter plot showing the extent of apoptosis in Lin− cells from the WT (left) and leukemic mice (right) on day 0 and after 6 days of drug treatment (vehicle, 0.1 mM and 1 mM pluripotin; 1 mM of gilteritinib). In each density plot quadrant show; Q1, Lin− cells (Lin+ and annexin V−); Q2, apoptosis of differentiated cells (annexin V+ and Lin+); Q3, apoptosis in Lin− cells (Lin− and annexin V+); and Q4, Lin− viable cells (annexin V− and Lin−). (B) Bar plot showing total apoptotic cells from both Lin− and Lin+ cells. (C) Bar plots showing the apoptotic cells from Lin− cells. (D) Bar plot representing apoptotic cells from Lin+ cells. (E) Bar plots showing annexin V+ cells in CD34+ cells from the normal and AML donors before and after 6 days of drug treatment. (F) Bar plot depicting the colony-forming unit (CFU) numbers from the normal and AML CD34 cells treated with vehicle (dimethyl sulfoxide), pluripotin (0.1 mM and 1 mM) and gilteritinib (1 μM). All data presented are shown as mean ± SD collected from 2 independent experiments. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

Pluripotin induces apoptosis in leukemic cells while enriching the normal HSC progenitors. (A) Scatter plot showing the extent of apoptosis in Lin cells from the WT (left) and leukemic mice (right) on day 0 and after 6 days of drug treatment (vehicle, 0.1 mM and 1 mM pluripotin; 1 mM of gilteritinib). In each density plot quadrant show; Q1, Lin cells (Lin+ and annexin V); Q2, apoptosis of differentiated cells (annexin V+ and Lin+); Q3, apoptosis in Lin cells (Lin and annexin V+); and Q4, Lin viable cells (annexin V and Lin). (B) Bar plot showing total apoptotic cells from both Lin and Lin+ cells. (C) Bar plots showing the apoptotic cells from Lin cells. (D) Bar plot representing apoptotic cells from Lin+ cells. (E) Bar plots showing annexin V+ cells in CD34+ cells from the normal and AML donors before and after 6 days of drug treatment. (F) Bar plot depicting the colony-forming unit (CFU) numbers from the normal and AML CD34 cells treated with vehicle (dimethyl sulfoxide), pluripotin (0.1 mM and 1 mM) and gilteritinib (1 μM). All data presented are shown as mean ± SD collected from 2 independent experiments. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

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