Inhibition of ATL cell growth by DNA demethylating agents and EZH2 inhibitors in vitro. (A) MT-1 and MT-2 cells were treated with each compound alone or in combination for 4 days, and cell growth was assessed using the CCK-8 reagent. Synergy scores were calculated using the SynergyFinder software.32 ZIP Synergy scores −10 to 10 indicate additive, >10 indicate synergistic, and less than −10 indicate antagonistic effects.33 (B) MT-1, MT-2, and TL-Om1 cells were cultured for 4 days with DNA demethylating agents (DAC or OR21) in the presence or absence of EZH2 inhibitors (EPZ or DS). Cell growth was assessed using the CCK-8 reagent. The absorbance of untreated cells was defined as 100%. The results are expressed as the mean of 3 independent experiments ± SD. Differences between groups were tested using the Tukey test. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. n.s., not significant; SD, standard deviation.
