Cyclin D1 is associated with IFN signatures and immune exhaustion in EVI1-AML. (A) GSEA showing that gene sets associated with chemokine activity are downregulated after silencing Ccnd1 in vitro. (B) GSEA showing that gene sets associated with IFN-γ signaling are upregulated by silencing Ccnd1 in vivo. (C) qPCR showing the relative expression of Stat1 in EVI1-AML cells with Ccnd1 silencing. (D) qPCR showing the relative expression of exhaustion-related genes in the spleen T cells from shLuc- or shCcnd1-EVI1-AML mice before the development of full-blown AML. Freshly isolated AML cells (1 × 10⁶) from shLuc- or shCcnd1-EVI1-AML mice were injected into the secondary recipient mice without TBI, followed by T-cell isolation from the spleen 19 days after transplant. Please see supplemental Figure 6H. (E) Frequency of cellular subsets of CD4 T cells infiltrating the liver of EVI1-AML mice with frank leukemia. Please see supplemental Figure 6I. (F) Frequency of cellular subsets of CD8 T cells infiltrating the liver of EVI1-AML mice with indicated shRNA after exposure to 4.5 Gy TBI. Please see supplemental Figure 6I. (G-H) Pearson correlation analysis between IFN-γ score and MECOM/CCND1 expression in TCGA samples. (I) Pearson correlation analysis between STAT1 and CCND1 expression in TCGA samples. (J) Pearson correlation analysis between CD274 and CCND1 expression in TCGA samples. Mean ± SD. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001.