MyeloVec fully restores HD levels of gp91^phox and cellular oxidase in human X-CGD HSPC-derived neutrophils. HSPCs from patients with X-CGD were transduced with MyeloVec or CCLchim and differentiated into mature neutrophils in vitro. (A) Restoration of gp91^phox was measured at day 18 of differentiation. (B) Oxidase activity of the mature neutrophils was measured by DHR flow cytometry. (C) MFI of the entire neutrophil population is shown. (D) MFI of the DHR⁺ neutrophils is shown. (E) Production of reactive oxygen species was measured by the cytochrome C assay. Data are presented as mean ± SD. Statistical significance was analyzed using an unpaired t test. All statistical tests were 2-tailed and P < .05 was deemed significant; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001. ctrl, control.