Survival of TP53^mut t-MN is poor irrespective of the allelic status or bone marrow blast percentage. (A) Proportion of TP53^mut t-MN patients with TP53 VAF ≤10% or >10% or loss of TP53 locus without TP53^mut. Of the 15 patients with VAF ≤10%, 13 had VAF ≤10% without loss of heterozygosity (LOH). Two patients had LOH across the TP53 locus and were grouped with “loss of TP53 locus” (n = 10). (B) OS of TP53^mut with VAF >10% or loss of TP53 locus was significantly poor compared with TP53^wt and TP53^mut with VAF ≤10% t-MN. (C) The frequency of TP53^mut subgroup within the TP53^mut t-MN. TP53^mut subgroup were defined as cases with single mutation (1 mut), ≥2 mutations without the loss of chromosome 17p13 across TP53 locus (≥2 mut), mutation(s) plus copy neutral LOH (cnLOH) and/or loss of the TP53 locus (mut + loss). (D) Number of patients with 0, 1, or ≥2 TP53^mut. Colors represent the status of chromosome 17 at the TP53 locus, to include cnLOH, loss of TP53 locus, and no detected aberration (normal). Unbalanced translocations leading to loss of TP53 locus are encoded as “loss.” OS of single- vs multi-hit TP53^mut in the whole cohort (E), t-MDS (F), t-AML (G), and bone marrow blast 0% to 9% (H).