Phagocytes and a unicellular organism share a CEBPα-driven phagocytic program. (A,D) Venn diagrams with the number of highly expressed genes (A) and TFs (D) in Capsaspora or mouse macrophages compared with mouse ESCs and nonphagocytic blood cells. (B) Top 8 KEGG pathways involved in the 11 genes highly expressed in Capsaspora and mouse macrophages compared with mouse ESCs and nonphagocytic blood cells. (C) PC analyses of various lineages or stages of 4 species: Capsaspora, sponge, tunicate, and mouse. Expression levels of 62 conserved TFs were compared. (E) Heatmap of scaled expression levels (z score) of TFs in Capsaspora, mouse macrophages, mouse ESCs, and mouse nonphagocytic blood cells. Four TFs expressed higher in Capsaspora or mouse macrophages than in mouse ESCs and nonphagocytic blood cells were selected. Expression levels were scaled among the 8 cell groups. (F) Expression levels of CEBPα homologs and PLA2G15 homologs in Capsaspora, and various mouse cell lineages. Data are mean ± standard error of the mean. Statistical significance of differences between 3 stages of Capsaspora are shown, ∗P < .05, ∗∗P < .01. KEGG, Kyoto Encyclopedia of Genes and Genomes.