Heterogeneity in both normal and malignant SS T cells. (A) Heatmap of the top 10 genes for each of the single-cell clusters with Bonferroni P value < .05. Bars on the left indicate malignant (red) or nonmalignant (blue) predominance. (B) Density plots of the principal component analysis (PC1 and PC2) of genes involved in T-cell differentiation. (C) Selection of genes by cluster identity for skin-homing, central memory, and Treg phenotypes (IL7R-low/negative associated with Tregs). (D) Percent of cells in each cluster in phases of the cell cycle by regression analysis. (E) ssGSEA enrichment scores for gene signatures derived from the malignant SS clusters applied to a mixed MF/SS bulk RNA cohort.²⁴ (F) The proportion of dominant V read vs ssGSEA enrichment scores for gene signatures derived from the malignant SS clusters applied to a mixed MF/SS bulk RNA cohort.²⁴ (G) Z-score scaled ssGSEA enrichment scores for Biocarta, Kegg, and Hallmark libraries by single-cell cluster. The median values across nonmalignant (NM) clusters are reported in a single column.