Meis1 upregulates stem cell- and cell cycle–associated pathways in Npm1 haploinsufficient cells. (A) Venn diagram of RNA-seq data comparing differentially expressed genes in NPM1^+/− plus Meis1 vs NPM1^+/+ plus Meis1; both cohorts were first compared with their respective empty vector controls. (B) GSEA analysis for pathways upregulated in NPM1^+/− plus Meis1 vs NPM1^+/+ plus Meis1 cells. (C) Heatmap of differentially expressed genes in NPM1^+/− plus Meis1, Hoxa9 plus Meis1 (H9M1), and AML1-ETO9a (AE9A) primary leukemic mice.^31,32 (D) GSEA analysis for pathways enriched in NPM1^+/− plus Meis1 vs H9M1 and AE9A primary leukemic mice. ∗P < .05; ∗∗P < .001; ∗∗∗P < .0001. FDR, false discovery rate; NES, normalized enrichment score.