Figure 2.
Npm1 haploinsufficiency and Meis1 overexpression collaborate to induce AML. (A) Schematic of the experimental design of in vivo experiments with Npm1. (B) Kaplan-Meier plot of NPM1+/+ mice transduced with a MIY empty vector (n = 2), NPM1+/− plus MIY (n = 5), NPM1+/+ plus Meis1 (n = 7), NPM1+/− plus Meis1 (n = 10), secondary transplants of NPM1+/− plus Meis1 leukemic BM (n = 7), and tertiary transplants of NPM1+/− plus Meis1 leukemic BM (n = 3). (C) Histopathological analysis of a NPM1+/− plus Meis1 mouse. (D) Colony-forming assay of NPM1+/− plus Meis1 ex vivo cells (NM1) transduced with scrambled control or shRNA against Meis1 (n = 4). indicates number of independent experiments. ∗P < .05; ∗∗P < .001; ∗∗∗P < .0001. MIY, MSCV IRES YFP plasmid; n, number of independent experiments.

Npm1 haploinsufficiency and Meis1 overexpression collaborate to induce AML. (A) Schematic of the experimental design of in vivo experiments with Npm1. (B) Kaplan-Meier plot of NPM1+/+ mice transduced with a MIY empty vector (n = 2), NPM1+/− plus MIY (n = 5), NPM1+/+ plus Meis1 (n = 7), NPM1+/− plus Meis1 (n = 10), secondary transplants of NPM1+/− plus Meis1 leukemic BM (n = 7), and tertiary transplants of NPM1+/− plus Meis1 leukemic BM (n = 3). (C) Histopathological analysis of a NPM1+/− plus Meis1 mouse. (D) Colony-forming assay of NPM1+/− plus Meis1 ex vivo cells (NM1) transduced with scrambled control or shRNA against Meis1 (n = 4). indicates number of independent experiments. ∗P < .05; ∗∗P < .001; ∗∗∗P < .0001. MIY, MSCV IRES YFP plasmid; n, number of independent experiments.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal