OTSSP167inhibitscell viability in T-ALL cell lines. (A) Expression of MELK and MAP2K7 kinases in T-ALL cell lines. (B) Cell viability assays in T-ALL cell lines treated with OTSSP167 for 48 hours (n=3). IC₅₀ is indicated for each cell line. Cell viability assays were averaged from 3 independent experiments performed in triplicates. (C) Potency of OTSSP167 in the panel of T-ALL cell lines. (D) Cytotoxicity in a leukemic cell line compared with an LCL (nonleukemic). (E) Comparative potency of JNK-IN-8, 5Z-7Oxozeaenol (5Z7O), and OTSSP167 in KOPT-K1 cells.