G-MDSCs improves hematopoiesis under irradiation-mediated BM suppression. (A) C.B10 mice were irradiated at 5 Gy (TBI, n = 6), some mice were treated with 10 × 10⁶ C.B10 BM G-MDSCs/mouse IV (TBI+Ly6G cells, n = 9), normal C.B10 mice were included as normal controls (CON, n = 4). (B) Complete blood counts and total BM number were evaluated at 2 weeks after irradiation. (C) Hematopoietic stem and progenitor cells in the BM. Flow cytometry plots were gated from Lin⁻ cells. Frequency and absolute number of KSL (CD117⁺Sca-1⁺Lin⁻) and myeloid progenitor cells (MP, CD117⁺Sca-1⁻Lin⁻) are displayed. Data are shown as means with standard errors. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.