Platelet-rich thrombosis model (FeCl₃ induced; rh-tPA resistant). (A) AIS was induced by topical application of FeCl₃ on the MCA. (B) Bar graph (mean ± standard deviation) displaying the percentage of reperfusion of the ischemic core, calculated as difference between levels of perfusion 10 minutes after treatment (T = 70 minutes) and 10 minutes before treatment (T = 10 minutes). From individual mice indicated as red circles, images are presented hereafter. (C) Representative laser speckle flow images (from subjects indicated at red circles in panel B) before and after treatment from a single mouse per group. Reperfusion seems to be absent as the blue and green hemisphere (bottom half of each image) before treatment remains blue and green after treatment. The mean CBF levels in the ischemic core (expressed as percent of the unaffected hemisphere) are shown below the images. (D) Bar graph (mean ± standard deviation) displaying lesion volumes, calculated from a T2-weighted MRI. From individual mice indicated as red circles (reflecting median values), images are presented hereafter. (E) Representative T2-weighted images of coronal mouse brain slices displaying the hyperintense lesion area in a single mouse with median lesion volume per group (indicated as red circles in panel D). Lesion volume values are shown below the images. Asterisks indicate significance levels as measured by Mann-Whitney U tests: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. CBF, cerebral blood flow.