Fibrin-rich thrombosis model (thrombin induced; rh-tPA sensitive). (A) AIS was induced by intravascular injection of thrombin into the MCA. Clot formation was considered to be stable when blood flow in the MCA was zero for 10 minutes. Cortical perfusion over the entire dorsal brain area was measured via speckle imaging between T = 10 minutes and T = 70 minutes. Treatment was given at T = 20 minutes as a 10% bolus and 90% infusion over 40 minutes. MRI was performed after 24 hours to assess lesion volumes (T2 weighted), possible hemorrhage (T2∗-weighted), and MCA recanalization (MR angiography). (B) Bar graph (mean ± standard deviation) displaying the percentage of reperfusion of the ischemic core, calculated as difference between levels of perfusion 10 minutes after treatment (T = 70 minutes) and 10 minutes before treatment (T = 10 minutes). From individual mice indicated as red circles (reflecting median values), images are presented hereafter. (C) Representative laser speckle flow images (from subjects indicated at red circles in panel B) before and after treatment from a single mouse per group. Reperfusion is visible when the blue hemisphere (bottom half of each image) before treatment is turning green, yellow, or red after treatment. The mean CBF levels in the ischemic core (expressed as percent of the unaffected hemisphere) are shown below the images. (D) Bar graph (mean ± standard deviation) displaying lesion volumes, calculated from a T2-weighted MRI. From individual mice indicated as red circles (reflecting median values), images are presented hereafter. (E) Representative T2-weighted images of coronal mouse brain slices displaying the hyperintense lesion area in a single mouse per group (indicated as red circles in D). Lesion volume values are shown below the images. Asterisks indicate significance levels as measured by Mann-Whitney U tests: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. CBF, cerebral blood flow.