Derivation, specificity, and functionality of iDuo NK cells. (A) iDuo NK cells are genetically engineered from iPSC-derived NK cells incorporating expression of a CD19-targeting CAR with NKG2D transmembrane and 2B4/CD3ζ signaling domains, a high-affinity, non-cleavable CD16, and a membrane-bound IL-15/IL-15R fusion molecule. (B) Triple modification allows for dual target-mediated killing through the CAR with enhanced ADCC when used in combination with monoclonal targeted antibody.