Prolonged XPO1 inhibition preferentially inhibits growth and induces differentiation of NPM1-mutated cell lines. (A) Viable cell counts by trypan blue exclusion of indicated NPM1-mutated and NPM1 WT cell lines treated with 50 nM eltanexor for 11 days. Cells were counted and replated at equal concentrations with fresh drugs every 3 days. Results are reported as ratio to DMSO-treated cells. N = 3, mean ± SEM, Dunnett multiple comparison test. (B) Flow cytometry quantification of CD11b, expressed as MFI FC relative to DMSO in OCI-AML3 and indicated NPM1 WT cell lines after 11 days of treatment with eltanexor 50 nM 5 days per week. N = 3, mean ± SEM, Sidak multiple comparison test for each cell line. (C) Cell cycle analysis by propidium iodide of OCI-AML3 and indicated NPM1 WT cell lines after 11 days of treatment with eltanexor 50 nM 5 days per week. N = 3, mean ± SEM, Tukey multiple comparison test.