Differentiation induced by prolonged XPO1 inhibition is marginally dependent on TP53. (A) Immunoblot analysis of p53 in WT and TP53^KO OCI-AML3 clones 15 and 17. (B) Flow cytometry quantification of CD11b, expressed as percentage of CD11b⁺ cells in WT and TP53^KO OCI-AML3 clones after 7 and 12 days of CT with selinexor 50 nM. N = 6, mean ± SEM, Dunnett multiple comparison test. (C) Flow cytometry quantification of CD11b, expressed as MFI FC relative to DMSO in WT and TP53^KO OCI-AML3 clones after 7 and 12 days of CT with selinexor 50 nM. N = 6, mean ± SEM, Dunnett multiple comparison test. (D) Histogram plot showing CD11b expression in WT and TP53^KO OCI-AML3 clones after 7 and 12 days of CT with DMSO or selinexor 50 nM. (E) Immunoblot analysis of p53 protein expression in WT and TP53^KO dTAG OCI-AML3 clones 3 and 22. (F) Flow cytometry quantification of CD11b, expressed as percentage of CD11b⁺ cells in WT and TP53^KO dTAG OCI-AML3 clones after 7 and 12 days of CT with selinexor 50 nM. N = 6, mean ± SEM, Dunnett multiple comparison test. (G) Flow cytometry quantification of CD11b, expressed as MFI FC relative to DMSO in WT and TP53^KO dTAG OCI-AML3 clones after 7 and 12 days of CT with selinexor 50 nM. N = 6, mean ± SEM, Dunnett multiple comparison test. (H) Histogram plot showing CD11b expression in WT and TP53^KO dTAG OCI-AML3 clones after 7 and 12 days of CT with DMSO or selinexor 50 nM. cl, clone.