FVII-mediated FX activation determined by chromogenic assay (2-step FXa generation) and the ability of FVII variants to support plasma thrombin generation in FVII-deficient plasma. (A) FVII from each variant was diluted to 500 pM and used to evaluate coagulant activity. Data were reported as FVII activity (mean ± SEM, n = 3). (B) Representative thrombin generation curves showing the ability of FVII variants to support TF-triggered thrombin generation. (C) The mean peak thrombin and (D) ETP values of all FVII variants were similar to those of WT. (E) Lag time and (F) time-to-peak values of FVII variants were significantly longer than those of WT. The results were presented as mean ± SEM (n = 3). ∗∗∗P < .001; ∗∗P < .01; ∗P < .05 in comparison between WT and each FVII variant.