Expression of ER stress–related genes. ATF4 (A), BiP (B), CHOP (C), XBP1 (D), and ATF6 (E) were determined in each FVII variant–transfected HEK293T by qRT-PCR. The results were presented as mean ± SEM (n = 3). ∗∗∗P < .001; ∗∗P < .01 in comparison between WT and each FVII variant.