Intracellular localization of FVII variants. Intracellular localization of FVII variants in the ER (A) and the Golgi (B). The markers of cell organelles (ER and Golgi) were stained in red color. FVII was stained in green. Colocalization of FVII with a cell organelle marker (ER or Golgi) was shown in yellow color. Scale bars, 10 μm. The colocalization of FVII with PDI (C) and GM130 (D) was calculated by the PCC. The PCC score was estimated from 10 cells for each FVII. ∗∗∗P < .001; ∗∗P < .01; ∗P < .05 in comparison between WT and each FVII variant.