SPAG6, in association with MYO1D, promotes cell growth and migration of AML cells. (A-B) K562 and HEL cells with SPAG6 knockdown and HL-60 and THP-1 cells with SPAG6 overexpression were transfected with MYO1D forced expression vector or small interfering RNA as indicated. The cell proliferative capacities were detected by CCK-8 and EdU assays in K562, HEL, HL-60, and THP-1 cells with treatment as indicated. (C) Transwell assay in K562, HEL, HL-60, and THP-1 cells treated as indicated. (D) K562 cells with SPAG6 knockdown were transfected with MYO1D forced expression or control vector as indicated. Kaplan-Meier curves showed the survival of mice treated as indicated. (E-F) Size and weight of the spleen between different treatment groups. (G) White blood cells (WBCs) in mice were treated as indicated. *P < .05; **P < .01.