SPAG6 activates the PI3K/AKT and ERK signaling pathways in a MYO1D-dependent manner. (A) Western blot showing EGFR, ERBB2, ERBB3, and ERBB4 expression in K562 and HEL cells with MYO1D knockdown. (B-C) The proliferative capacities of K562 and HEL cells with MYO1D knockdown were detected by CCK-8 and EdU assays. (D) Migration of K562 and HEL cells when MYO1D was knocked down. (E-F) Western blot showing the concentrations of EGFR, ERBB2, ERBB3, ERBB4, AKT, p-AKT, ERK, and p-ERK in 4 AML cells treated as indicated. (G-H) Western blot showing the concentrations of AKT, p-AKT, ERK, and p-ERK in K562, HEL, HL-60, and THP-1 cells with treatment as indicated. *P < .05; **P < .01.