Figure 5.
Detection of eGFP- or tdTomato-tagged iPSC-derived cells by genomic PCR in collected postmortem tissues. Peripheral blood mononuclear cells from animal undergoing transplantation with eGFP-transduced HSPCs were used as positive control for animals that received eGFP-tagged iHPs. Tissues were collected 25 days (cy0959) or 1 year (remaining animals) after iHP infusion. Day-10 iHPs expressing tdTomato were used as positive control for animals that received iHPs tagged with tdTomato.

Detection of eGFP- or tdTomato-tagged iPSC-derived cells by genomic PCR in collected postmortem tissues. Peripheral blood mononuclear cells from animal undergoing transplantation with eGFP-transduced HSPCs were used as positive control for animals that received eGFP-tagged iHPs. Tissues were collected 25 days (cy0959) or 1 year (remaining animals) after iHP infusion. Day-10 iHPs expressing tdTomato were used as positive control for animals that received iHPs tagged with tdTomato.

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