Grab is upregulated in differentiating erythroblasts. (A) Relative expression of Grab and control genes from RNA sequencing analyses on differentiating MEL cells (left)²² and mouse primary fetal liver erythroblasts (MPFE, right).²⁵ MEL cells before (uninduced) and after DMSO-induced differentiation were analyzed. R1 to R5 represent the progressive stages of differentiating erythroblasts. (B-C) qRT-PCR (B) and immunoblotting (C) analyses of Grab in differentiating mouse fetal liver erythroblasts. Erythropoietin (Epo) was supplemented to the erythroid progenitors isolated from the livers of E14.5 mouse embryos to induce the erythroid differentiation. Tfrc and Gapdh were used as controls. *P < .05. (D-E) qRT-PCR (D) and immunoblotting (E) analyses of Grab in MEL cells before and after DMSO-induced erythroid-like differentiation. Tfrc and Tubulin were used as controls. **P < .01. (F-G) qRT-PCR (F) and immunoblotting (G) analyses of GRAB in K562 cells before and after sodium butyrate (NaB)-induced erythroid-like differentiation. TFRC and ACTIN were used as controls. **P < .01. (H-I) Western analyses of Grab in multiple mouse tissues (H) and human cell lines (I).