Figure 4.
TFIs maintain high T-cell metabolic fitness. (A) Kinetic plot and corresponding bar graphs of normalized OCR obtained during mitochondrial stress test of T cells continuously stimulated with AMG 562; n = 3. (B) Kinetic plot and corresponding bar graphs of normalized OCR obtained during mitochondrial stress test of T cells after 14 days of continuous vs TFI AMG 562 stimulation; n = 5. (C) Kinetic plot and corresponding bar graphs of normalized ECAR obtained during glycolysis stress test of T cells after 14 days of continuous vs TFI AMG 562 stimulation; n = 5. All graphs present mean ± SEM values. Statistical analysis: Kruskal–Wallis and Dunn’s multiple comparison test (A); 2-way ANOVA and Sidak’s multiple comparison test (B-C); *P < .05; **P < .01. ECAR, extracellular acidification rate; OCR, oxygen consumption rate; ±SEM, standard error of the mean; TFI(s), treatment-free interval(s).

TFIs maintain high T-cell metabolic fitness. (A) Kinetic plot and corresponding bar graphs of normalized OCR obtained during mitochondrial stress test of T cells continuously stimulated with AMG 562; n = 3. (B) Kinetic plot and corresponding bar graphs of normalized OCR obtained during mitochondrial stress test of T cells after 14 days of continuous vs TFI AMG 562 stimulation; n = 5. (C) Kinetic plot and corresponding bar graphs of normalized ECAR obtained during glycolysis stress test of T cells after 14 days of continuous vs TFI AMG 562 stimulation; n = 5. All graphs present mean ± SEM values. Statistical analysis: Kruskal–Wallis and Dunn’s multiple comparison test (A); 2-way ANOVA and Sidak’s multiple comparison test (B-C); *P < .05; **P < .01. ECAR, extracellular acidification rate; OCR, oxygen consumption rate; ±SEM, standard error of the mean; TFI(s), treatment-free interval(s).

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