Aberrant cytoplasmic localization of NPM1e5 mutations. (A) NPM1 exon 5 protein sequences. Rectangles indicate amino acids insertions, and predicted NES are indicated in red. (B) NPM1 localization in primary NPM1 wild-type (WT) and NPM1 mutated samples. (C) Overexpression of WT and mutated patient-specific sequences in HEK-293t cell lines. Images were acquired on a Leica DMi8 microscope using 100× magnification with oil immersion. Brightness and contrast were adjusted with ImageJ.