Effects of venetoclax in DHL cells. (A-C) Trypan blue exclusion test of cell viability in CARNAVAL (A), WILL-2 (B), and Oci-Ly7 (C) cells treated with increasing concentrations of venetoclax (VEN) for up to 48 hours. Technical triplicates were counted per experiment. Graphs show results of 3 independent experiments (n = 3, SD). (D-F) Annexin V staining measured by flow cytometry in CARNAVAL (D), WILL-2 (E), and Oci-Ly7 (F) cells subjected to escalating concentrations of 1 nM to 1 µM VEN and DMSO for 12 hours. (G-I) Protein expression of Bcl-2 and apoptotic markers Caspase 3, 7, 9, cPARP, and total levels analyzed by Western blot in CARNAVAL (G), WILL-2 (H), and Oci-Ly7 (I) cells after treatment with VEN for 12 hours with concentrations as indicated. Tubulin served as a loading control. Quantification of Western blot was done with ImageJ. Intensities were calculated relative to tubulin and normalized to DMSO solvent control. N/A, not analyzable.