Inhibitors of Hedgehog signaling impair thrombus formation in mice. (A) Representative timelapse images exhibiting thrombus formation in mice, preadministered either with vehicle (control), cyclopamine, or vismodegib, captured 5, 15, or 25 minutes after injury of mesenteric arterioles of >100 μm diameter with 10% ferric chloride. (B-E) Scatter dot plots representing (B) time to first thrombus formation, (C) time to occlusion, (D) thrombus growth rate, and (E) tail-bleeding time in mice preadministered with vehicle, cyclopamine, or vismodegib. Each dot in scatter plots represents an independent observation. (F) Kaplan-Meier curve exhibiting proportion of occluded arteries at various time points of observation in mice preadministered with vehicle, cyclopamine, or vismodegib. Data are expressed as the mean ± standard error of the mean. *P < .05 with respect to vehicle-treated mice.