Phage library screen discovers an ALNEQIARL-specific scFv with efficacy against various cancer cell lines in CAR T-cell format. (A) Phage library screen procedure. (B) Characterization of six NDC80 CAR T-cell clones (ie, A-F) against peptide pulsed T2 cells. (C) Efficacy and specificity of NDC80-clC CAR T cells against hematologic cancer cell lines. (D) Efficacy and specificity of NDC80-clC CAR T cells against nonhematologic cancer cell lines. Error bars in panels B through D denote standard deviation. FACS, fluorescence-activated cell sorter; E:T, effector-to-target ratio.