Vamifeport prevented organ iron loading in the Townes model of SCD (HbSS mice). Mice received water supplemented with the stable iron isotope ⁵⁸Fe to distinguish iron acquired during the treatment period and iron already present in organs. ⁵⁸Fe concentrations in the liver and spleen were measured by using inductively coupled plasma mass spectroscopy. (A) Vamifeport significantly reduced serum iron levels 3 hours postdose. Vamifeport prevented ⁵⁸Fe loading of livers (B) and spleens (C) of HbSS mice. (D) Vamifeport corrected total iron content in HbSS RBCs to the levels of HbAA mice. Results are presented as individual values with mean ± standard deviation (n = 7-10 mice per group). Analysis was performed by using one-way analysis of variance with Dunnett’s multiple comparison of all groups vs the HbSS vehicle group: ***P < .001. BID, twice daily.