Vamifeport decreased PS exposure and improved mitochondria clearance in mature RBCs of HbSS mice. (A) Vamifeport lowered the exposure of PS on peripheral blood RBCs, as detected by a decreased percentage of Annexin V–positive cells and decreased intensity of Annexin V staining. (B) Although almost all reticulocytes of vehicle- and vamifeport-treated HbSS mice contained mitochondria (left), treatment with vamifeport reduced the occurrence of mitochondria in mature RBCs (right). Results are presented as individual values with mean ± standard deviation (n = 7-10 mice per group). Analysis was performed by using one-way analysis of variance with Dunnett’s multiple comparison of all groups vs the HbSS vehicle group: *P < .05, ***P < .001. BID, twice daily; MFI, mean fluorescent intensity.