Quercetin inhibits NLRP3 inflammasome priming, IL-1beta secretion, and TNF-α production in XIAP-deficient cells. Four strains of mouse BMDMs were preincubated with quercetin before stimulation with either CpG ODN or TNF-α. We then measured the levels of (A) IL-1β, (B) TNF-α, and (C) cell loss. Quercetin reduced the secreted levels of IL-1β and TNF-α. Quercetin reduced levels of (D) ROS (normalized to control), and (E) inhibited upregulation of NLRP3 and pro–IL-1β following CpG ODN or TNF-α priming. Data for lipopolysaccharide (LPS)-treated cells are also shown in block E. Horizontal black lines on Western blot images indicate that bands of different molecular weights from the original probe and subsequent reprobes with antibodies against the indicated proteins were cropped together. Symbols used: n.s for not significant, * for p≤0.05, and ** for p≤0.01.