Disorganization of FRC/FDC networks and increase of CD68⁺ macrophages in CLL lymph nodes. (A) Representative images from nontumoral (panels a-d) and CLL (panels e-h) lymph node tissue sections. Immunohistochemical staining was used on samples (anti-CCL21 and anti-αSMA or anti-CXCL13 and anti-CD21 antibodies), representative of FRC and FDC (original magnification ×200). (B) Representative images from nontumoral (left panels) and CLL (right panels) lymph node tissue sections stained with anti-CD68 antibody (original magnification ×200 and ×400, respectively). (C) Quantification of CD68⁺ cells per 550 μm² section in nontumoral (n = 10) and CLL lymph nodes (n = 10); statistical analysis was carried out by Student t-test (*P < .005). Images were acquired using a DFC 300 FX Leica microscope with a ×10 objective.