Effects of IBR treatment on in vitro CD40 and BCR stimulation. (A) As an indirect way to study LN metabolism, PB mononuclear cell samples from patients with CLL at baseline or after 3-month treatment with IBR were restimulated with in vitro CD40/BCR by coculture on CD40L-expressing 3T3 cells (CD40) or by anti-IgM–coated beads (BCR) for 24 hours. Metabolomic analysis was subsequently performed in these samples. (B) CD95 was measured after 24 hours of CD40 stimulation, and pS6 was measured after 30-minute BCR stimulation. Representative histograms are shown for all conditions. (C) Percentages of CD95-high cells and pS6-high cells were calculated (n = 8). Data are shown as mean ± standard error of the mean of the indicated individual values. Statistical analyses were performed with paired Student t test. *P < .05, **P < .01, ***P < .001. ns, not significant.