Progenitor exhausted T-cell genes upregulated in THRLBCL. (A) The stacked area chart displays the gene expression value of 13 TIS genes in the THRLBCL (purple) and DLBCL (light blue) cases of the discovery set. (B) Circos plot shows the overlap between the 2 gene lists: the list of genes upregulated in THRLBL vs DLBCL (in blue) and the list of genes upregulated in progenitor exhausted T cells vs terminally exhausted T cells (in red). The 2 gene lists are on the outside (red/blue); the orange circle on the inside represents genes that were unique to each gene list, and the dark orange represents genes shared by the 2 gene lists. Purple lines link the same genes shared by the 2 lists. The names of the shared genes are reported in the purple triangle. Box plot histograms represent the expression value of TCF7/TCF1 gene in terms of log₂ normalized gene counts in THRLBCL vs DLBCL samples analyzed by using the NanoString immune gene panel in the discovery set (C) and in the in silico set (D). (E) Box plot histograms represented the percentage of cells positive for TCF1 protein by IHC. (F) CD3 and TCF1 immunostaining showed higher expression in THRLBCL vs DLBCL cases (Olympus BX53 light microscope, Olympus DP71 camera; original magnification, 60×). Statistical significance was calculated by using the Mann-Whitney U test.