Exhausted related genes upregulated in THRLBCL vs DLBCL. (A) Circular dendrogram of the exhausted related genes upregulated in THRLBCL vs DLBCL cases of the discovery set (created with RAWGraphs). The IC genes are colored in light purple; the virus-induced exhausted related genes, according to the in silico data (accession number GSE9650),¹⁷  in light blue; and the EOMES upregulated genes in light green. The gene circle size is proportional to the FC gene expression value. The asterisks indicate the genes shared by 2 categories. (B) Box plot histograms represent the expression value of PD-1, PD-L1, LAG3, and EOMES genes in terms of gene counts (normalized and log₂-transformed) in the THRLBCL vs DLBCL samples analyzed in the discovery set by using NanoString immune gene panel. (C) Box plot histograms represent the expression value of PD-1, PD-L1, LAG3, and EOMES genes in terms of gene counts (normalized and log₂-transformed) in the THRLBCL vs DLBCL samples of the in silico set. (D) Box plot histograms represent the percentage of cells positive for the immunostaining of PD-1, PD-L1, LAG3, and EOMES proteins in the IHC validation set samples. (E) PD-1, PD-L1, LAG3, and EOMES immunostaining showed higher expression in THRLBCL vs DLBCL cases (Olympus BX53 light microscope, Olympus DP71 camera; original magnification, 40×). Statistical significance was calculated by using the Mann-Whitney U test.