Figure 1.
Intrinsic characteristics of apheresed T cells and quality of the CAR T-cell product. (A) Representative staining of naïve/stem cell memory (TN/SCM; CD45RO− CD27+), central memory (TCM; CD45RO+CD27+), effector memory (TEM; CD45RO+CD27−), and effector (TEFF; CD45RO−CD27−) CD8 and CD4 subsets in apheresed T lymphocytes. (B) Distribution of TN/SCM, TCM, TEM, and TEFF CD8 and CD4 T cells in apheresed T lymphocytes from 30 patients treated with tisa-cel. Bars represent the median. (C) Correlation between the naïve/stem cell memory phenotype and in vitro proliferative capacity (proportion of CFSElow cells) of apheresed CD8 (left panel) and CD4 (right panel) T cells. (D) The CD4/CD8 T-cell ratio in the final product correlates with the ratio of CD4 TN/SCM to CD8 TN/SCM cells in the apheresis (left) and the ratio of CFSElow CD4 to CD8 T cells (right). Pearson correlation test. ρ coefficient (R) and P values are indicated.

Intrinsic characteristics of apheresed T cells and quality of the CAR T-cell product. (A) Representative staining of naïve/stem cell memory (TN/SCM; CD45RO CD27+), central memory (TCM; CD45RO+CD27+), effector memory (TEM; CD45RO+CD27), and effector (TEFF; CD45ROCD27) CD8 and CD4 subsets in apheresed T lymphocytes. (B) Distribution of TN/SCM, TCM, TEM, and TEFF CD8 and CD4 T cells in apheresed T lymphocytes from 30 patients treated with tisa-cel. Bars represent the median. (C) Correlation between the naïve/stem cell memory phenotype and in vitro proliferative capacity (proportion of CFSElow cells) of apheresed CD8 (left panel) and CD4 (right panel) T cells. (D) The CD4/CD8 T-cell ratio in the final product correlates with the ratio of CD4 TN/SCM to CD8 TN/SCM cells in the apheresis (left) and the ratio of CFSElow CD4 to CD8 T cells (right). Pearson correlation test. ρ coefficient (R) and P values are indicated.

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