Abnormal WPB distribution in primary ECs derived from endothelium-specific E1841K mutant mice. (A) Immunostaining of NMII-A (green) and actin (red) in BECs from cre-, PF4-cre, and Tie2-cre/E1841K^flox/flox mice. Scale bars represent 5 μm. (B) Immunostaining of VWF (white) and actin (red) in these BECs. (C) Percentage of WPBs in the perinuclear area (n = 16; NS > 0.05; **P < .01). NS, not significant.