Generation and characterization of platelet-specific deletion of GRK2 in mouse. (A) Schematic map of loxP sites inserted in the mouse GRK2 gene. Two loxP sites flanking exons III through VI of mouse GRK2 are shown as triangles. (B) Western blot images of platelet lysate from PF4-Cre^– and PF4-Cre⁺ mice probed with the GRK2-, GRK5-, and GRK6-specific antibodies. Deletion of GRK2 in platelets does not affect the expression of GRK5 and GRK6. n = 4. Supplemental Figure 2 features the full blots. ns, not significant.