Analysis of transduction in peripheral blood cells. (A) VCN per cell in PBMCs (black lines) and total blood cells (RBCs+PBMCs, red lines, measured only for HDAd5/35++GFP/cxcr4– and HDAd5/3+GFP/cxcr4–injected animals). (B) Analysis of transgene mRNA levels relative to mRNA levels of the housekeeping gene GAPDH. Human mgmt^P140K mRNA data are shown by black lines; human cxcr4 mRNA levels are shown by red lines. Note that the scale of the y-axis for the HDAd5/3+GFP/cxcr4–injected animal is different. (C) Percentage of GFP⁺ HSCs in peripheral blood white cells. The scale of the y-axis for the HDAd5/3+GFP/cxcr4–injected animal is different.