LysM-Atg5^−/− mice had normal hepatic histology but mild fibrosis. (A) Histogram of the mean and standard error values with dot plots of liver weight normalized to body weight (bw; n = 14-15 in each group). (B) Representative liver anatomy of LysM-Atg5^−/− and WT mice. Immunofluorescence of liver sections stained with a phycoerythrin-conjugated anti-F4/80 antibody (red) and 4′,6-diamidino-2-phenylindole (DAPI; blue) for nuclear staining showing similar Kupffer cell distribution (Leica fluorescence microcopy imaging; original magnification, 20×; scale bars, 20 μm). Liver sections stained with hematoxylin and eosin (H&E) and Sirius red with Fast green counterstaining (NanoZoomer scan imaging; original magnification, 20×; scale bars, 50 μm); Sirius red⁺ areas of collagen (red) are indicative of mild liver fibrosis in LysM-Atg5^−/− mouse livers. Histologic staining panels are representative of LysM-Atg5^−/− and WT mice (n = 8 per group). (C) Histogram of the mean and standard error values with dot plots of serum AST levels (n = 12-15 in each group). *P < .05.