Platelet function and GPVI expression decrease during platelet storage. Quantification of GPVI (A), P-selectin expression of platelets stimulated with CRP-XL 5 µg/mL (B). (C) Immature platelet fraction. Platelet deposition (D), Thrombus multilayer formation (E) and P-selectin expression (F), each given as a percent surface area coverage (SAC). Platelets were reconstituted in platelet- and leukocyte-depleted whole blood perfused over collagen-coated surfaces. Platelet concentrates were prepared from buffy coats, split and stored at room temperature (RT, red lines) or 4°C (blue lines). n ≥ 4. *P < .05, **P < .01. Blue and red asterisks represent significant changes over time; black asterisks represent significant differences between storage conditions.