NfκB dynamics change throughout the hematopoietic hierarchy. (A) Main NfκB response dynamics type frequencies in different cell types (manual classification). (B-E) Cell type–specific signaling dynamics. (B) Single-cell heat stripes (vertical) of all measured cells and conditions. Brightness represents nuclear p65 signal intensity (n = 2721 cells: 166 HSC, 167 MPP1, 93 MPP2, 340 MPP3, 210 PreGM, 680 GMP, 329 Mas, 204 PreMegE, and 290 MkP; N = 3 biological replicates). The first 6 hours are shown (12 hours in supplemental Figure 2H). (C) Frequencies of main response types per cell type (manual classification). Mean ± standard deviation (3 independent replicates). P values in supplemental Figure 2G. Colors as in (A). (D) UMAP representation based on extracted signaling dynamics features. UMAP shape as in Figure 1D. Only TNFα-stimulated cells are shown. Green dots = cell type as indicated in (B). (E-F) Three cell type–specific signaling dynamics feature scores across cell types (E) and on the signaling dynamics UMAP representation (F). UMAP shape as in Figure 1E. See also supplemental Figure 2.