Extended knob-hole interactions affect overall visco-elastic properties of the clot (tanδ). Micro-rheology experiments were conducted by forming a fibrin clot in a capillary tube. Tan[δ] at 0.1 Hz (A) was significantly increased for γDEK, whereas the single variants were similar to WT. At 1 Hz (B) and 10 Hz (C), [tanδ] was similar to WT for all variants. Mean ± SD, N = 3. ***P < .001 compared with WT.