Generation and characterization of ST3GAL1 and/or ST3GAL2 KO iPSC lines. (A) Schematic illustration of the ST3GAL1 and ST3GAL2 locus showing the location of the gRNA binding sites (red arrows) to guide Cas9 to its cleavage site. ATG start codon for gene translation is marked by the green arrow. (B) Western blot demonstrating the loss of expression of ST3GAL1 and/or ST3GAL2 in corresponding KO iPSC lines. (C) Flowcytometric analysis demonstrating the expression of SSEA3, SSEA4, and TRA 1-60 surface markers on different iPSC lines. Blue boxes highlight the carbohydrate epitopes recognized by anti-SSEA3 and anti-SSEA4 antibodies.