Expression of ST3GAL1 and ST3GAL2 during MK and EB differentiation from human iPSCs. (A) Schematic for generating MKs and EBs from iPSCs. (B) Flow cytometric analysis showed production of CD34⁺/CD43⁺ HPCs, CD41⁺/CD42b⁺ MKs, and CD71⁺/CD235ab⁺ EBs from SSEA3⁺/SSEA4⁺ iPSCs. (C) Western blot showed the expression of ST3GAL1 and ST3GAL2 at different differentiation stages.